概述

  • 产品名称
  • 描述
    兔多克隆抗体to PURA
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Sheep
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PURA.

    (Peptide available as ab88160.)

  • 阳性对照
    • This antibody gave a positive signal in Human Brain Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab79936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
  • 序列相似性
    Belongs to the PUR DNA-binding protein family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PUR ALPHA antibody
    • PUR1 antibody
    • purA antibody
    • PURA_HUMAN antibody
    • PURALPHA antibody
    • Purine rich element binding protein A antibody
    • Purine rich single stranded DNA binding protein alpha antibody
    • Purine-rich single-stranded DNA-binding protein alpha antibody
    • Transcriptional activator protein PUR alpha antibody
    • Transcriptional activator protein Pur-alpha antibody
    see all

图片

  • Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.

  • ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.

文献

This product has been referenced in:

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Cell lysate - whole cell (whole brain)
Specification
whole brain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 10 2013

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed agains...

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Thank you for contacting Abcam. I am sorry that you have been experiencing difficulties with our two antibodies, ab14744 and ab77734.  We have received positive feedback for both of these antibodies in ICC and so I am not sure why they have not wor...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Username

Abcam user community

Verified customer

提交于 Jun 02 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,25% Triton in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 30 2010

Application
Western blot
Sample
Rat Tissue lysate - whole (brain lysate differentially centrifuged)
Loading amount
5 µg
Specification
brain lysate differentially centrifuged
Gel Running Conditions
Reduced Denaturing (12 % SDS Gel)
Blocking step
Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2010

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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