重组
RabMAb

Anti-Pumilio 1抗体[EPR3795] (ab92545)

概述

  • 产品名称
    Anti-Pumilio 1抗体[EPR3795]
    参阅全部 Pumilio 1 一抗
  • 描述
    兔单克隆抗体[EPR3795] to Pumilio 1
  • 特异性
    This antibody is predicted to not cross react with Pumilio 2.
  • 经测试应用
    适用于: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide. within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
    Database link: Q14671

  • 阳性对照
    • WB: HeLa and 293T cell lysates IHC-P: Human fetal kidney tissue ICC/IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab92545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 126 kDa.
IP 1/50.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

靶标

Anti-Pumilio 1 antibody [EPR3795] 图像

  • All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 126 kDa
  • Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.
  • Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.
  • Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Pumilio 1 antibody [EPR3795] (ab92545)参考文献

ab92545 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C antibody (sc-15386) bound protein A dynabeads 0.1 mg/ml (antibody/beads).
Negative control
Protein A dynabeads without antibody bound.
Immuno-precipitation step
Other - Protein A dynabeads
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提交于 Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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