重组
RabMAb

Anti-PU.1/Spi1抗体[EPR3158Y] (ab76543)

概述

  • 产品名称
    Anti-PU.1/Spi1抗体[EPR3158Y]
    参阅全部 PU.1/Spi1 一抗
  • 描述
    兔单克隆抗体[EPR3158Y] to PU.1/Spi1
  • 经测试应用
    适用于: IHC-Fr, IHC-P, WB, Flow Cytmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human PU.1/Spi1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P17947

  • 阳性对照
    • Daudi and U937 cell lysates. Human tonsil tissue.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000 - 1/20000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for ICC/IF or IP.
  • 靶标

    • 功能
      Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • 序列相似性
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    图片

    • ab76543 showing positive staining in Normal spleen tissue.

    • ab76543, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.
    • All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilution

      Lane 1 : Daudi cell lysates
      Lane 2 : U937 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    • ab76543 showing positive staining in Normal lung lymphocytes staining tissue.

    • ab76543 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Mohr S  et al. Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia. Cancer Cell 31:549-562.e11 (2017). IHC . Read more (PubMed: 28399410) »
    • Frank CL  et al. HDAC inhibitors cause site-specific chromatin remodeling at PU.1-bound enhancers in K562 cells. Epigenetics Chromatin 9:15 (2016). WB ; Human . Read more (PubMed: 27087856) »

    See all 3 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse prefrontal cortex)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% BisTris)
    Loading amount
    20 µg
    Specification
    Mouse prefrontal cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Sample
    Human Tissue sections (Lymph node)
    Specification
    Lymph node
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Jul 15 2013

    DISCOUNT CODE: 42636
    Expiration date:15-Mar-13

    I am very pleased to hear a customer would like to accept our offer and test ab76543 in IHC-Fr. To redeem this offer, an Abreview needs to be submitted for IHC-Fr on human samples and this cod...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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