Anti-PU.1/Spi1抗体(ab83399)

概述

  • 产品名称
    Anti-PU.1/Spi1抗体
    参阅全部 PU.1/Spi1 一抗
  • 描述
    兔多克隆抗体to PU.1/Spi1
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (QKGNRKKMTY QKMARALRNY GKTGEVKKVK KKLTYQFSGE VLGRGGLAER) of human PU.1/Spi1 (NP_003111).

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
  • 序列相似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • transcription factor spi1 antibody
    • 31 kDa Transforming Protein antibody
    • 31 kDa-transforming protein antibody
    • cb1086 antibody
    • Hematopoietic transcription factor PU.1 antibody
    • OF antibody
    • oncogene spi1 antibody
    • PU.1 antibody
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
    • SFPI1 antibody
    • si:by184l24.2 antibody
    • SPI 1 antibody
    • SPI 1 proto oncogene antibody
    • SPI A antibody
    • Spi1 antibody
    • SPI1_HUMAN antibody
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
    • Spleen focus forming virus proviral integration oncogene spi1 antibody
    • Transcription factor PU.1 antibody
    see all

图片

  • Anti-PU.1/Spi1 antibody (ab83399) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

实验方案

文献

ab83399 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Axolotl limb 30 days post amputation)
Permeabilization
Yes - Dako
Specification
Axolotl limb 30 days post amputation
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
Fixative
Acetone
Username

Crystal Jones

Verified customer

提交于 Nov 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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