概述

  • 产品名称Anti-PU.1/Spi1抗体
    参阅全部 PU.1/Spi1 一抗
  • 描述
    兔多克隆抗体to PU.1/Spi1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (QKGNRKKMTY QKMARALRNY GKTGEVKKVK KKLTYQFSGE VLGRGGLAER) of human PU.1/Spi1 (NP_003111).

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • transcription factor spi1 antibody
    • 31 kDa Transforming Protein antibody
    • 31 kDa-transforming protein antibody
    • cb1086 antibody
    • Hematopoietic transcription factor PU.1 antibody
    • OF antibody
    • oncogene spi1 antibody
    • PU.1 antibody
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
    • SFPI1 antibody
    • si:by184l24.2 antibody
    • SPI 1 antibody
    • SPI 1 proto oncogene antibody
    • SPI A antibody
    • Spi1 antibody
    • SPI1_HUMAN antibody
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
    • Spleen focus forming virus proviral integration oncogene spi1 antibody
    • Transcription factor PU.1 antibody
    see all

Anti-PU.1/Spi1 antibody 图像

  • Anti-PU.1/Spi1 antibody (ab83399) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

Anti-PU.1/Spi1 antibody (ab83399)参考文献

ab83399 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83399.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"