The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Add 100ul/well when tested in ELISA against the recombinant fragment used as the immunogen. We have no data on the detection of endogenous protein.
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
功能Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
序列相似性Belongs to the ETS family. Contains 1 ETS DNA-binding domain.
Ab66059 was coated on to an ELISA plate at a concentration of 0.01 mg/ml. Afer blocking, the immunogen (recombinant tagged fragment) was added to the wells at a range of concentrations (0.01-1000 ng/ml). A tag specific secondary antibody was added for detection at a concentration of 1 µg/ml.
Western blot - PU.1/Spi1 antibody (ab66059)
Anti-PU.1/Spi1 antibody (ab66059) at 1/500 dilution + Immunogen (recombinant fragment) at 0.2 µg
Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution developed using the ECL technique