Anti-PU.1/Spi1抗体(ab56173)

概述

  • 产品名称Anti-PU.1/Spi1抗体
    参阅全部 PU.1/Spi1 一抗
  • 描述
    兔多克隆抗体to PU.1/Spi1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide corresponding to N-terminal residues of mouse PU.1/Spi1

性能

应用

Our Abpromise guarantee covers the use of ab56173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • transcription factor spi1 antibody
    • 31 kDa Transforming Protein antibody
    • 31 kDa-transforming protein antibody
    • cb1086 antibody
    • Hematopoietic transcription factor PU.1 antibody
    • OF antibody
    • oncogene spi1 antibody
    • PU.1 antibody
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
    • SFPI1 antibody
    • si:by184l24.2 antibody
    • SPI 1 antibody
    • SPI 1 proto oncogene antibody
    • SPI A antibody
    • Spi1 antibody
    • SPI1_HUMAN antibody
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
    • Spleen focus forming virus proviral integration oncogene spi1 antibody
    • Transcription factor PU.1 antibody
    see all

Anti-PU.1/Spi1 antibody (ab56173)参考文献

ab56173 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step Real-time PCR
Positive control i) competitor antibody known to work
ii) a regultaory region known to bind this transcription factor
Negative control a regulatory region which is an open region of chromatin but devoid of this transcription factor
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提交于 Oct 06 2009

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