RabMAb

Anti-PTS (phospho S19)抗体[EPR1517(2)] (ab109462)

概述

  • 产品名称Anti-PTS (phospho S19)抗体[EPR1517(2)]
    参阅全部 PTS 一抗
  • 描述
    兔单克隆抗体[EPR1517(2)] to PTS (phospho S19)
  • 特异性ab109462 only detects PTS phosphorylated at Serine 19.
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 19 of Human PTS.

  • 阳性对照
    • HUVEC cell lysates; Human brain tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
    • 通路Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
    • 疾病相关Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.
    • 序列相似性Belongs to the PTPS family.
    • 翻译后修饰Phosphorylation of Ser-19 is required for maximal enzyme activity.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 6 pyruvoyl tetrahydrobiopterin synthase antibody
      • 6 pyruvoyl tetrahydropterin synthase antibody
      • 6 pyruvoyltetrahydropterin synthase antibody
      • 6-pyruvoyl tetrahydrobiopterin synthase antibody
      • EC 4.2.3.12 antibody
      • FLJ97081 antibody
      • OTTHUMP00000235385 antibody
      • PTP synthase antibody
      • PTPS antibody
      • PTPS_HUMAN antibody
      • PTS antibody
      see all

    Anti-PTS (phospho S19) antibody [EPR1517(2)] 图像

    • All lanes : Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution

      Lane 1 : HUVEC cell lysates
      Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 16 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109462, at a 1/250 dilution, staining PTS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109462, at a 1/250 dilution, staining PTS (phospho S19) in HeLa cells by Immunofluorescence.

    Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462)参考文献

    ab109462 has not yet been referenced specifically in any publications.

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