概述
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产品名称Anti-PTS (phospho S19)抗体[EPR1517(2)]
参阅全部 PTS 一抗 -
描述兔单克隆抗体[EPR1517(2)] to PTS (phospho S19)
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特异性ab109462 only detects PTS phosphorylated at Serine 19.
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经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性与反应: Human
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免疫原
A phospho specific peptide corresponding to residues surrounding Serine 19 of Human PTS.
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阳性对照
- HUVEC cell lysates; Human brain tissue; HeLa cells
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR1517(2)
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab109462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. | |
| ICC/IF | 1/250 - 1/500. |
靶标
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功能Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
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通路Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
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疾病相关Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.
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序列相似性Belongs to the PTPS family.
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翻译后修饰Phosphorylation of Ser-19 is required for maximal enzyme activity.
- Information by UniProt
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数据库链接
- Entrez Gene: 5805 Human
- GenBank: NP_000308.1 Human
- Omim: 612719 Human
- SwissProt: Q03393 Human
- Unigene: 503860 Human
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别名
- 6 pyruvoyl tetrahydrobiopterin synthase antibody
- 6 pyruvoyl tetrahydropterin synthase antibody
- 6 pyruvoyltetrahydropterin synthase antibody
see all
Anti-PTS (phospho S19) antibody [EPR1517(2)] 图像
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![Western blot - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)](http://www.abcam.cn/ps/datasheet/images/109/ab109462/PTS-Primary-antibodies-ab109462-1.JPG)
Western blot - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)All lanes : Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 16 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)](http://www.abcam.cn/ps/datasheet/images/109/ab109462/PTS-Primary-antibodies-ab109462-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)](http://www.abcam.cn/ps/datasheet/images/109/ab109462/PTS-Primary-antibodies-ab109462-3.JPG)
Immunocytochemistry/ Immunofluorescence - PTS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS (phospho S19) in HeLa cells by Immunofluorescence.
实验方案
Anti-PTS (phospho S19) antibody [EPR1517(2)] (ab109462)参考文献
ab109462 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"