概述

  • 产品名称Anti-PTRH2抗体
    参阅全部 PTRH2 一抗
  • 描述
    兔多克隆抗体to PTRH2
  • 特异性This antibody is specific for PTRH2.
  • 经测试应用适用于: WB, IHC (PFA fixed), ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the amino terminus of human PTRH2.

  • 阳性对照
    • A20 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab36990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC (PFA fixed)
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 2 µg/ml.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa). Can be blocked with PTRH2 peptide (ab39732).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
      Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
    • 序列相似性Belongs to the PTH2 family.
    • 细胞定位Mitochondrion.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Bcl 2 inhibitor of transcription 1 antibody
      • Bcl-2 inhibitor of transcription 1 antibody
      • BIT 1 antibody
      • BIT1 antibody
      • CGI 147 antibody
      • CGI147 antibody
      • mitochondrial antibody
      • Peptidyl tRNA hydrolase 2 mitochondrial antibody
      • Peptidyl-tRNA hydrolase 2 antibody
      • PTH 2 antibody
      • PTH2 antibody
      • PTH2_HUMAN antibody
      • PTRH 2 antibody
      • PTRH2 antibody
      see all

    Anti-PTRH2 antibody 图像

    • Lane 1 : Anti-PTRH2 antibody (ab36990) at 1 µg/ml
      Lane 2 : Anti-PTRH2 antibody (ab36990) at 2 µg/ml
      Lane 3 : Anti-PTRH2 antibody (ab36990) at 4 µg/ml

      Lane 1 : A20 cell lysate.
      Lane 2 : A20 cell lysate.
      Lane 3 : A20 cell lysate.

      Lysates/proteins at 15 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/10000 dilution

      Predicted band size : 20 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      The arrow next to the image points to the PTRH2 band.
    • ab36990 at 2µg/ml staining PTRH2 in 3T3 cells by ICC/IF

    • Immunofluorescence of Bit1 in 3T3 cells using ab36990 at 20 ug/ml.

    Anti-PTRH2 antibody (ab36990)参考文献

    This product has been referenced in:
    • Duthie KA  et al. Proteomics analysis of interleukin (IL)-7-induced signaling effectors shows selective changes in IL-7Ralpha449F knock-in T cell progenitors. Mol Cell Proteomics 6:1700-10 (2007). WB ; Mouse . Read more (PubMed: 17602185) »

    See 1 Publication for this product

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