概述

  • 产品名称Anti-PTRF抗体
    参阅全部 PTRF 一抗
  • 描述
    兔多克隆抗体to PTRF
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PTRF.

    (Peptide available as ab90995.)

  • 阳性对照
    • This antibody gave a positive signal in the following mouse whole cell lysates: NIH3T3; MEF1.

性能

应用

Our Abpromise guarantee covers the use of ab88213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
  • 疾病相关Congenital generalized lipodystrophy 4
  • 序列相似性Belongs to the PTRF/SDPR family.
  • 翻译后修饰Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
    Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
  • 细胞定位Membrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310075E07Rik antibody
    • AW546441 antibody
    • Cavin 1 antibody
    • Cavin antibody
    • Cavin-1 antibody
    • CAVIN1 antibody
    • CGL4 antibody
    • FKSG13 antibody
    • FLJ90031 antibody
    • MGC118550 antibody
    • OTTMUSP00000002049 antibody
    • Polymerase I and transcript release factor antibody
    • Ptrf antibody
    • PTRF_HUMAN antibody
    • RNA polymerase I and transcript release factor antibody
    • RP23-279L23.6 antibody
    • TTF I interacting peptide 12 antibody
    see all

Anti-PTRF antibody 图像

  • All lanes : Anti-PTRF antibody (ab88213) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ICC/IF image of ab88213 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab88213, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal Goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-PTRF antibody (ab88213)参考文献

ab88213 has not yet been referenced specifically in any publications.

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