概述

  • 产品名称Anti-PTRF抗体
    参阅全部 PTRF 一抗
  • 描述
    兔多克隆抗体to PTRF
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Opossum, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 125 and 175 of human PTRF (NP_036364.2)

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab76919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
  • 疾病相关Congenital generalized lipodystrophy 4
  • 序列相似性Belongs to the PTRF/SDPR family.
  • 翻译后修饰Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
    Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
  • 细胞定位Membrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310075E07Rik antibody
    • AW546441 antibody
    • Cavin 1 antibody
    • Cavin antibody
    • Cavin-1 antibody
    • CAVIN1 antibody
    • CGL4 antibody
    • FKSG13 antibody
    • FLJ90031 antibody
    • MGC118550 antibody
    • OTTMUSP00000002049 antibody
    • Polymerase I and transcript release factor antibody
    • Ptrf antibody
    • PTRF_HUMAN antibody
    • RNA polymerase I and transcript release factor antibody
    • RP23-279L23.6 antibody
    • TTF I interacting peptide 12 antibody
    see all

Anti-PTRF antibody 图像

  • All lanes : Anti-PTRF antibody (ab76919) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 43 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Western Blot of Immunprecipitate.
    ab76919 at 1µg/ml (for WB) staining PTRF in HeLa whole cell lysate,
    immunoprecipitated using ab76919 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-PTRF antibody (ab76919)参考文献

ab76919 has not yet been referenced specifically in any publications.

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