概述

  • 产品名称Anti-PTPN12抗体
    参阅全部 PTPN12 一抗
  • 描述
    兔多克隆抗体to PTPN12
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 760 and 780 of human PTPN12 (NP_002826.2)

  • 阳性对照
    • HeLa whole cell lysate and mouse NIH3T3 whole cell lysate A549 cell line IF/ICC

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab76942 was affinity purified using an epitope specific to PTPN12 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-PTPN12 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and colon carcinoma (right) tissues labelling PTPN12 with ab76942 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PTPN12 antibody (ab76942) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Lane 1: ab76942, at 1 µg/mg, staining PTPN12 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab76942 at 3µg/mg of lysate.
    Lane 2: Control IgG.
    Detection: Chemiluminescence with an exposure time of 3 seconds.
  • ICC/IF image of ab76942 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PTPN12 antibody (ab76942)参考文献

ab76942 has not yet been referenced specifically in any publications.

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