Anti-PTPN12抗体(ab76942)
Key features and details
- Rabbit polyclonal to PTPN12
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PTPN12抗体
参阅全部 PTPN12 一抗 -
描述
兔多克隆抗体to PTPN12 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to a region between residue 760 and 780 of human PTPN12 (NP_002826.2)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab76942 was affinity purified using an epitope specific to PTPN12 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 88 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 531877 Cow
- Entrez Gene: 5782 Human
- Entrez Gene: 19248 Mouse
- Entrez Gene: 117255 Rat
- Omim: 600079 Human
- SwissProt: Q05209 Human
- SwissProt: P35831 Mouse
- SwissProt: Q63745 Rat
see all -
别名
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and colon carcinoma (right) tissues labelling PTPN12 with ab76942 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-PTPN12 antibody (ab76942) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Lane 1: ab76942, at 1 µg/mg, staining PTPN12 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab76942 at 3µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76942 被引用在 4 文献中.
- Lemmens M et al. Evaluation of two in vitro assays for tumorigenicity assessment of CRISPR-Cas9 genome-edited cells. Mol Ther Methods Clin Dev 23:241-253 (2021). PubMed: 34703845
- Chen Z et al. PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells. Cancer Res 78:3809-3822 (2018). PubMed: 29743287
- Zhangyuan G et al. Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma. Cell Physiol Biochem 46:2335-2346 (2018). PubMed: 29742497
- Sztacho M et al. BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts. PLoS One 11:e0164829 (2016). WB, ICC/IF . PubMed: 27760174