概述

  • 产品名称Anti-PTPN12抗体
    参阅全部 PTPN12 一抗
  • 描述
    兔多克隆抗体to PTPN12
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 375 and 425 of human protein PTPN12 (NP_002826.2)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab76936 was affinity purified using an epitope specific to PTPN12 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5 ug/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 88 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Protein tyrosine phosphatase G1 antibody
      • Protein tyrosine phosphatase non receptor type 12 antibody
      • Protein-tyrosine phosphatase G1 antibody
      • PTN12_HUMAN antibody
      • PTP PEST antibody
      • PTP-PEST antibody
      • PTPG1 antibody
      • Ptpn12 antibody
      • Tyrosine protein phosphatase non receptor type 12 antibody
      • Tyrosine-protein phosphatase non-receptor type 12 antibody
      see all

    Anti-PTPN12 antibody 图像

    • All lanes : Anti-PTPN12 antibody (ab76936) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 88 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab76936 at 1 ug/ml (used at 3µg/mg lysate for IP).
      Lane 2: control IgG.
      Detection: Chemiluminescence with an exposure time of 1 second.

    Anti-PTPN12 antibody (ab76936)参考文献

    ab76936 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76936.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"