Anti-PTPLAD2抗体(ab82949)

概述

  • 产品名称
    Anti-PTPLAD2抗体
  • 描述
    兔多克隆抗体to PTPLAD2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (LLHIYVGIES NHLLPRFLQL TERIIILFVV ITSQEEVQEK YVVCVLFVFW) of human PTPLAD2 (NP_001010915).

  • 阳性对照
    • 293T cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-PTPLAD2 antibody (ab82949) at 1 µg/ml (in 5% skim milk / PBS) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 66 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab82949 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tissue microarray with several normal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA buffer, pH 9.0
Permeabilization
No
Specification
Tissue microarray with several normal tissues
Blocking step
Blocking agent from Novolink Max Polymer Detection System as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde
Username

Lucas Bidinotto

Verified customer

提交于 Mar 11 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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