Anti-PTP4A1抗体(ab1368)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PTP4A1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Peptide with sequence KDSNGHRNNCCIQ, from C-Terminus of the protein sequence according to NP_003454

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: 1/2000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa in HeLa and A549 lysates (predicted molecular weight: 20 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.
    • 组织特异性
      Expressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines.
    • 序列相似性
      Belongs to the protein-tyrosine phosphatase family.
      Contains 1 tyrosine-protein phosphatase domain.
    • 发展阶段
      Expressed in fetal liver.
    • 翻译后修饰
      Farnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus.
    • 细胞定位
      Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HH 72 antibody
      • HH72 antibody
      • Hypothetical protein DKFZp779M0721 antibody
      • Phosphatase of regenerating liver 1 antibody
      • PRL 1 antibody
      • PRL-1 antibody
      • PRL1 antibody
      • Protein tyrosine phosphatase PTPCAAX1 antibody
      • Protein tyrosine phosphatase type IVA 1 antibody
      • Protein tyrosine phosphatase type IVA member 1 antibody
      • Protein-tyrosine phosphatase 4a1 antibody
      • Protein-tyrosine phosphatase of regenerating liver 1 antibody
      • Protein-tyrosine phosphatase, type 4A, 1 antibody
      • PTP(CAAXI) antibody
      • Ptp4a1 antibody
      • PTPCAAX1 antibody
      • TP4A1_HUMAN antibody
      see all

    Anti-PTP4A1 antibody 图像

    • Anti-PTP4A1 antibody (ab1368) at 2 µg/ml + Human A549 lysate (35µg in RIPA buffer)

      Predicted band size : 20 kDa

    实验方案

    Anti-PTP4A1 antibody (ab1368)参考文献

    ab1368 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (COS7 extract expressing HA-tagged PTP4A1.)
    Loading amount
    10 µg
    Specification
    COS7 extract expressing HA-tagged PTP4A1.
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Blocking One as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 09 2009

    Thank you for your enquiry. We have withdrawn ab1368from our product range. We do hope that this does not cause you too much inconvenience. Have you purchased this antibody? Have you done any experiments with it?

    Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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