RabMAb

Anti-PTP1B (phospho S50)抗体[EP1838Y] (ab62320)

概述

  • 产品名称
    Anti-PTP1B (phospho S50)抗体[EP1838Y]
    参阅全部 PTP1B 一抗
  • 描述
    兔单克隆抗体[EP1838Y] to PTP1B (phospho S50)
  • 特异性
    This antibody detects PTP1B when phosphorylated at Serine 50.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B.

  • 阳性对照
    • Hela cell lysate.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能
    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻译后修饰
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

图片

  • All lanes : Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320) at 1/5000 dilution

    Lane 1 : Hela cell lysate - untreated cells
    Lane 2 : Hela cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

实验方案

文献

ab62320 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab62320.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册