Anti-PTP1B (phospho S50)抗体(ab88472)

概述

  • 产品名称
    Anti-PTP1B (phospho S50)抗体
    参阅全部 PTP1B 一抗
  • 描述
    兔多克隆抗体to PTP1B (phospho S50)
  • 特异性
    ab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to amino acids surrounding Ser 50 of Human PTP1B

  • 阳性对照
    • Lysate from Human A431 and Jurkat cells treated with Calyculin A.

性能

应用

Our Abpromise guarantee covers the use of ab88472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 序列相似性
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻译后修饰
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 细胞定位
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    图片

    • All lanes : Anti-PTP1B (phospho S50) antibody (ab88472) at 1/1000 dilution

      Lane 1 : lysate from untreated Jurkat cells
      Lane 2 : lysate from Jurkat cells treated with calyculin A (100 nM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    实验方案

    文献

    ab88472 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (muscle cells)
    Loading amount
    40 µg
    Specification
    muscle cells
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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