RabMAb

Anti-PTP1B (phospho S378)抗体[EP1840Y] (ab76239)

概述

  • 产品名称Anti-PTP1B (phospho S378)抗体[EP1840Y]
    参阅全部 PTP1B 一抗
  • 描述
    兔单克隆抗体[EP1840Y] to PTP1B (phospho S378)
  • 特异性ab76239 detects PTP1B phosphorylated on serine 378.
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding serine 378 of human PTP1B

  • 阳性对照
    • HeLa cytoplasmic or nuclear lysate treated with calyculin A Human breast carcinoma tissue
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
  • 应用说明ICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
    WB: 1/500000 - 1/1000000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻译后修饰Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho S378) antibody [EP1840Y] 图像

    • All lanes : Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239) at 1/1000000 dilution

      Lane 1 : HeLa cell lysates - untreated
      Lane 2 : HeLa cell lysates - treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab76239, at a 1/50 dilution, staining human PTP1B (phospho S378) in breast carcinoma by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239)参考文献

    ab76239 has not yet been referenced specifically in any publications.

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