概述

  • 产品名称Anti-PTP1B抗体[EP1841Y]
    参阅全部 PTP1B 一抗
  • 描述
    兔单克隆抗体[EP1841Y] to PTP1B
  • 特异性ab75856 detects both phosphorylated and nonphosphorylated PTP1B.
  • 经测试应用适用于: WB, ICC, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding serine 386 of human PTP1B.

  • 阳性对照
    • HeLa, HepG2 or Jurkat cell lysates. Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab75856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻译后修饰Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 细胞定位Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B antibody [EP1841Y] 图像

    • ab75856, at 1/50 dilution, staining PTP1B in human tonsil, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.


    • Predicted band size : 50 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PTPB1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab75856 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab75856 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab75856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-PTP1B antibody [EP1841Y] (ab75856) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    Anti-PTP1B antibody [EP1841Y] (ab75856)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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