Anti-PTP rho抗体[T20-AF3C7] (ab85333)

概述

  • 产品名称
    Anti-PTP rho抗体[T20-AF3C7]
    参阅全部 PTP rho 一抗
  • 描述
    小鼠单克隆抗体[T20-AF3C7] to PTP rho
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 887-1169 of Human PTP rho purified from E.coli.

  • 阳性对照
    • transfected Bosc23 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    Purified from TCS
  • 克隆
    单克隆
  • 克隆编号
    T20-AF3C7
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 164 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能
    May be involved in both signal transduction and cellular adhesion in the CNS.
  • 组织特异性
    Expressed in colon, lung, heart and testis, as well as in fetal and adult brain. Not detected in muscle and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein tyrosine phosphatase receptor type T antibody
    • PTPRT antibody
    • PTPRT_HUMAN antibody
    • R PTP T antibody
    • R-PTP-T antibody
    • Receptor protein tyrosine phosphatase antibody
    • Receptor-type tyrosine-protein phosphatase rho antibody
    • Receptor-type tyrosine-protein phosphatase T antibody
    • RPTP-rho antibody
    • RPTPmam4 antibody
    • RPTPrho antibody
    see all

图片

  • Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



    All lanes : Anti-NF-kB p65 (phospho S529) antibody (ab84333) at 1/5000 dilution

    Lane 1 : 293T Cell Lysate
    Lane 2 : Precipitated from 293T Cell Lysate 200ug using ab84333 2ug
    Lane 3 : Precipitated from 293T Cell Lysate 200ug using ab84333 5ug
    Lane 4 : Precipitated from PBS using ab84333 5ug
  • All lanes : Anti-PTP rho antibody [T20-AF3C7] (ab85333) at 1/10000 dilution

    Lane 1 : Bosc23 Cell Lysate
    Lane 2 : Bosc23 Cell Lysate Transfected with Myc/DUSP12
    Lane 3 : Bosc23 Cell Lysate Transfected with Myc/R-PTP rho
    Lane 4 : Bosc23 Cell Lysate Transfected with GFP/Ub

    Predicted band size: 164 kDa



    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-PTP rho antibody [T20-AF3C7] (ab85333) at 1/5000 dilution

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau (1-441 aa)
    Lane 3 : Bosc23 cell lysate transfected with Myc/His/PTP rho (877-1169 aa)
    Lane 4 : Bosc23 cell lysate transfected with GFP-Ub+1

    Predicted band size: 164 kDa



    The observed band size may not correspond to the predicted protein molecular weight as a recombinant fragment was used for the test lane.

文献

ab85333 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry and your interest in our product.
I can confirm that the immunogen used to raise this antibody was a recombinant fragment, corresponding to amino acids 887-1169 of Human PTP rho purified from E.coli.
I hope this hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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