WB: Inconclusive data due to lysates used. A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230), however no band is observed in human brain or pancreas lysate.
IF/ICC: see figure below.
Tested in peptide ELISA.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilution
Lane 1 : Human brain lysate Lane 2 : Human kidney lysate Lane 3 : Human pancreas lysate Lane 4 : Human heart lysate Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Exposure time : 5 minutes
A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
Image courtesy of www.proteinatlas.org ab13223 staining PTP lambda in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Anti-PTP lambda antibody (ab13223)参考文献
has not yet been referenced specifically in any publications.