概述

  • 产品名称
    Anti-PTP kappa抗体
    参阅全部 PTP kappa 一抗
  • 描述
    兔多克隆抗体to PTP kappa
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide derived from residues 900 - 1000 of Human PTP kappa.

    参阅Abcam的专有抗源政策

    .

  • 阳性对照
    • This antibody gave a positive result in PTP beta (PTPRB) and PTP kappa (PTPRK) recombinant protein domains

性能

应用

Our Abpromise guarantee covers the use of ab13225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).

靶标

  • 功能
    Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa.
  • 组织特异性
    High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • 翻译后修饰
    This protein undergoes proteolytic processing.
  • 细胞定位
    Cell junction > adherens junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • dJ480J14.2.1 antibody
      • DKFZp686C2268 antibody
      • DKFZp779N1045 antibody
      • OTTHUMP00000017180 antibody
      • OTTHUMP00000017181 antibody
      • OTTHUMP00000017182 antibody
      • OTTHUMP00000017187 antibody
      • OTTHUMP00000040306 antibody
      • Protein tyrosine phosphatase kappa antibody
      • Protein tyrosine phosphatase kappa precursor antibody
      • Protein tyrosine phosphatase receptor type K antibody
      • Protein tyrosine phosphatase receptor type kappa antibody
      • Protein-tyrosine phosphatase kappa antibody
      • PTPK antibody
      • PTPkappa antibody
      • Ptprk antibody
      • PTPRK_HUMAN antibody
      • R PTP kappa antibody
      • R-PTP-kappa antibody
      • Receptor type tyrosine protein phosphatase kappa antibody
      • Receptor-type tyrosine-protein phosphatase kappa antibody
      see all

    图片

    • Western blot - Anti-PTP kappa antibody (ab13225)
      Western blot - Anti-PTP kappa antibody (ab13225)
      All lanes : Anti-PTP kappa antibody (ab13225) at 1 µg/ml

      Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
      Lane 2 : 0.1µg SAP 1 (PTPRH) protein
      Lane 3 : 0.1µg PTP gamma (PTPRG) protein
      Lane 4 : 0.1µg PTP beta (PTPRB) protein
      Lane 5 : 0.1µg DEP1 (PTPRJ) protein
      Lane 6 : 0.1µg PTP kappa (PTPRK) protein
      Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
      Lane 8 : 0.1µg LAR (PTPRF) protein

      Performed under reducing conditions.

      Predicted band size: 50-70 kDa
      Observed band size: 65 kDa (why is the actual band size different from the predicted?)



      Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000.  The antibody recognises the recombinant PTP kappa fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.

      Lane #         Recombinant Protein

      1                  GLEPP1 (PTP RO)
      2                  SAP 1 (PTP RH)
      3                  PTP gamma (PTP RG)
      4                  PTP beta (PTP B)
      5         

    实验方案

    文献

    This product has been referenced in:
    • Agarwal S  et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . Read more (PubMed: 23696788) »
    • Flavell JR  et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). Read more (PubMed: 17720884) »

    See all 2 Publications for this product

    发表研究结果有使用 ab13225？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    2 reviews or Q&A

    10
    Votes: Highest First

    Customer contacted us to inquire about the WB image and expected band size when using this product.

    Thank you for contacting us.

    I have been informed that this product is tested using recombinant PTP phosphatase domains. The expected size for these recombinant peptides is around ˜65kDa. We have not tested this in endogenous systems howe...

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    Inquiry: I am looking for an antibody that will work on Human PTPRK in an immunoblot and with FACS as well. Can you tell me if ab13225 will do the job? Does it bind inter or extracellularly?

    Thank you for your enquiry and your interest.

    This is a polyclonal antibody so it binds to different epitopes. Thus for polyclonal antibodies epitope mapping is usually not carried out.

    I can confirm that ab13225 has only been teste...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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