Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis.
All lanes : Anti-PTOP antibody (ab112050) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 5 µg Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes
Immunoprecipitation - PTOP antibody (ab112050)
ab112050 at 1 µg/ml staining PDE3A in HeLa cell lysate immunoprecipitated using ab112050 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: controle IgG.
Predicted band size : 58 kDa
Exposure time: 30 seconds