• 产品名称
  • 描述
    兔多克隆抗体to PTIP
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human PTIP (N terminal). Synthetic peptide mapping to a region between residues 1 and 50 of Human PTIP, using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569
    Database link: Q6ZW49-6

  • 阳性对照
    • Whole cell lysate from 293T cells



Our Abpromise guarantee covers the use of ab70434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/25000. Detects a band of approximately 130 kDa (predicted molecular weight: 118 kDa).
IP Use at 5-10 µg/mg of lysate.


  • 功能
    Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes.
  • 序列相似性
    Contains 6 BRCT domains.
  • 结构域
    The BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently.
  • 细胞定位
    Nucleus matrix. Localizes to DNA damage foci upon ionizing radiation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAGF 28 antibody
    • CAGF 29 antibody
    • CAGF28 antibody
    • CAGF29 antibody
    • FLJ41049 antibody
    • PACIP 1 antibody
    • PACIP1 antibody
    • PAX interacting (with transcription activation domain) protein 1 antibody
    • PAX interacting protein 1 antibody
    • PAX transactivation activation domain-interacting protein antibody
    • PAX transcription activation domain interacting protein 1 like antibody
    • PAX-interacting protein 1 antibody
    • PAXI1_HUMAN antibody
    • PAXIP 1 antibody
    • PAXIP 1L antibody
    • paxip1 antibody
    • PAXIP1 protein antibody
    • PAXIP1L antibody
    • Protein encoded by CAG trinucleotide repeats antibody
    • PTIP antibody
    • TNRC 2 antibody
    • TNRC2 antibody
    see all

Anti-PTIP antibody 图像

  • Lanes 1, 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/ml
    Lanes 2, 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/ml
    Lanes 3, 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 5 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from 293T cells at 5 µg
    Lane 5 : Whole cell lysate from 293T cells at 15 µg
    Lane 6 : Whole cell lysate from 293T cells at 50 µg
    Lane 7 : Whole cell lysate from 293T cells at 5 µg
    Lane 8 : Whole cell lysate from 293T cells at 15 µg
    Lane 9 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 118 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds
  • 30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.

Anti-PTIP antibody (ab70434)参考文献

ab70434 has not yet been referenced specifically in any publications.

Product Wall

TheSwissProt database entry for PTIP notes there are 5 isoforms of this protein, one of which is about 90kDa. Although its not clear what the smaller molecular weight bands are.

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