Anti-PTIP抗体(ab70434)
Key features and details
- Rabbit polyclonal to PTIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PTIP抗体
参阅全部 PTIP 一抗 -
描述
兔多克隆抗体to PTIP -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Pig, Chimpanzee, Orangutan -
免疫原
Synthetic peptide corresponding to Human PTIP (N terminal). Synthetic peptide mapping to a region between residues 1 and 50 of Human PTIP, using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569
Database link: Q6ZW49-6 -
阳性对照
- Whole cell lysate from 293T cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70434于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2500 - 1/25000. Detects a band of approximately 130 kDa (predicted molecular weight: 118 kDa).
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IP |
Use at 5-10 µg/mg of lysate.
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说明 |
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WB
1/2500 - 1/25000. Detects a band of approximately 130 kDa (predicted molecular weight: 118 kDa). |
IP
Use at 5-10 µg/mg of lysate. |
靶标
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功能
Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes. -
序列相似性
Contains 6 BRCT domains. -
结构域
The BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently. -
细胞定位
Nucleus matrix. Localizes to DNA damage foci upon ionizing radiation. - Information by UniProt
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数据库链接
- Entrez Gene: 22976 Human
- Omim: 608254 Human
- SwissProt: Q6ZW49 Human
- Unigene: 443881 Human
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别名
- CAGF 28 antibody
- CAGF 29 antibody
- CAGF28 antibody
see all
图片
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Lanes 1 & 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/ml
Lanes 2 & 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/ml
Lanes 3 & 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/ml
Lanes 1 & 4 & 7 : Whole cell lysate from 293T cells at 5 µg
Lanes 2 & 5 & 8 : Whole cell lysate from 293T cells at 15 µg
Lanes 3 & 6 & 9 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab70434 被引用在 4 文献中.
- Tang M et al. FOXK1 Participates in DNA Damage Response by Controlling 53BP1 Function. Cell Rep 32:108018 (2020). PubMed: 32783940
- Schoonen PM et al. Progression through mitosis promotes PARP inhibitor-induced cytotoxicity in homologous recombination-deficient cancer cells. Nat Commun 8:15981 (2017). PubMed: 28714471
- Xu Y et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart. Elife 6:N/A (2017). PubMed: 29106372
- Kantidakis T et al. Mutation of cancer driver MLL2 results in transcription stress and genome instability. Genes Dev 30:408-20 (2016). PubMed: 26883360