概述

  • 产品名称Anti-PTGER3抗体
    参阅全部 PTGER3 一抗
  • 描述
    兔多克隆抗体to PTGER3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide derived from Human EP3 Receptor.

  • 阳性对照
    • SGC and Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab117998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 43 kDa.

靶标

  • 功能Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems.
  • 组织特异性Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EP 3 antibody
    • EP3 antibody
    • EP3 I antibody
    • EP3 II antibody
    • EP3 III antibody
    • EP3 IV antibody
    • Ep3 prostanoid receptor antibody
    • Ep3 receptor antibody
    • EP3 subtype antibody
    • EP3e antibody
    • HGNC:9595 antibody
    • MGC141828 antibody
    • MGC141829 antibody
    • MGC27302 antibody
    • PE2R3_HUMAN antibody
    • PGE receptor EP3 subtype antibody
    • PGE2 R antibody
    • PGE2 receptor EP3 subtype antibody
    • PGE2-R antibody
    • PGE2R antibody
    • Pgerep3 antibody
    • Prostaglandin E receptor 3 (subtype EP3) antibody
    • Prostaglandin E receptor 3 antibody
    • Prostaglandin E receptor EP3 subtype 3 isoform antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin e2 receptor ep3 antibody
    • Prostaglandin E2 receptor EP3 subtype antibody
    • Prostaglandin receptor (PGE 2) antibody
    • Prostaglandin receptor 3, EP3, subtype antibody
    • Prostanoid EP3 receptor antibody
    • PTGER 3 antibody
    • Ptger3 antibody
    • Ptgerep3 antibody
    • Rep3 antibody
    • rEP3a antibody
    • rEP3b antibody
    see all

Anti-PTGER3 antibody 图像

  • All lanes : Anti-PTGER3 antibody (ab117998) at 1/2000 dilution

    Lane 1 : SGC cell lysate at 70 µg
    Lane 2 : ECL cell lysate at 75 µg
    Lane 3 : Jurkat cell lysate at 70 µg


    Predicted band size : 43 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-PTGER3 antibody (ab117998)参考文献

This product has been referenced in:
  • Wang X  et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog 12:e1005803 (2016). WB ; Mouse . Read more (PubMed: 27482714) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification macrophages
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 13 2016

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ou...

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Thank you for this information. I was curious about the samples as I have received a similar inquiry regarding mouse and rat.


For a membrane protein, it is strongly recommended to use a lysis buffer containing NP-40 or to use RIPA buffer....

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Thank you for your reply.


Are you also testing another sample besides rat (human or mouse)? Can you provide your sample preparation details? How much protein are you loading?


How long do you block? Incubate with primary anti...

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Thank you for contacting Abcam regarding ab117998.


I have confirmedwith the laboratory that the immunogen sequence is: XXX Please contact us for additional information.

This antibody shares 70% identity with the rat sequence (th...

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Thank you for contacting Abcam regarding ab117998.


I am sorry that you have been experiencing difficulties with this antibody in WB. I am currently in touch with the laboratory to get additional information regarding the immunogen sequenc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"