概述

  • 产品名称
  • 描述
    兔多克隆抗体to PTF1A
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Mouse PTF1A aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: Q9QX98
    (Peptide available as ab155818)

  • 阳性对照
    • This antibidy gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum.

性能

应用

Our Abpromise guarantee covers the use of ab125973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).

靶标

  • 功能
    Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
  • 组织特异性
    Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
  • 疾病相关
    Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069].
  • 序列相似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位
    Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.
  • Information by UniProt
  • 数据库链接
  • 形式
    PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.
  • 别名
    • bHLH transcription factor p48 antibody
    • bHLHa29 antibody
    • Class A basic helix-loop-helix protein 29 antibody
    • Class II bHLH protein PTF1A antibody
    • Exocrine pancreas specific transcription factor p48 antibody
    • p48 DNA binding subunit of transcription factor PTF1 antibody
    • p48 DNA-binding subunit of transcription factor PTF1 antibody
    • PACA antibody
    • PAGEN2 antibody
    • Pancreas specific transcription factor 1a antibody
    • Pancreas transcription factor 1 subunit alpha antibody
    • Pancreas-specific transcription factor 1a antibody
    • PTF 1A antibody
    • PTF1 p48 antibody
    • PTF1-p48 antibody
    • Ptf1a antibody
    • PTF1A_HUMAN antibody
    • PTF1P48 antibody
    see all

图片

  • All lanes : Anti-PTF1A antibody (ab125973) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Cerebellum Mouse Tissue Lysate
    Lane 4 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

实验方案

文献

ab125973 has not yet been referenced specifically in any publications.

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