Anti-PTF1A抗体(ab125973)
Key features and details
- Rabbit polyclonal to PTF1A
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PTF1A抗体
参阅全部 PTF1A 一抗 -
描述
兔多克隆抗体to PTF1A -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Chinese hamster -
免疫原
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阳性对照
- This antibidy gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125973于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa). |
靶标
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功能
Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development. -
组织特异性
Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers. -
疾病相关
Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069]. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
细胞定位
Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex. - Information by UniProt
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数据库链接
- Entrez Gene: 19213 Mouse
- Entrez Gene: 117034 Rat
- SwissProt: Q9QX98 Mouse
- SwissProt: Q64305 Rat
- Unigene: 69647 Mouse
- Unigene: 10536 Rat
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形式
PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas. -
别名
- bHLH transcription factor p48 antibody
- bHLHa29 antibody
- Class A basic helix-loop-helix protein 29 antibody
see all
图片
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All lanes : Anti-PTF1A antibody (ab125973) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The predicted molecular weight of PTF1A is 35 kDa (SwissProt), however we expect to observe a banding pattern around 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125973 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125973 尚未被引用在任何文献中。