Antioxidant and antiapoptotic agent; analog of resveratrol
生物学描述Antioxidant and antiapoptotic agent; analog of resveratrol (ab120726). Antineoplastic properties in vitro and in vivo.
存放说明Store at -20°C. Store at -20°C long term. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
溶解度概述Soluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - Pterostilbene (ab120961)
ab51031 staining beclin1 in MCF7 cells treated with pterostilbene (ab120961), by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature. The cells were incubated at 37°C for 48h in media containing different concentrations of ab120961 (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51031 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
This product has been referenced in:
Zhang L et al. Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo. Apoptosis17:25-36 (2012).
Read more (PubMed: 21928089) »
Park ES et al. Pterostilbene, a natural dimethylated analog of resveratrol, inhibits rat aortic vascular smooth muscle cell proliferation by blocking Akt-dependent pathway. Vascul Pharmacol53:61-7 (0).
Read more (PubMed: 20398797) »