Anti-PTCHD1抗体(ab109407)

概述

  • 产品名称
    Anti-PTCHD1抗体
  • 描述
    山羊多克隆抗体to PTCHD1
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-DFQKTSRVSER

    , corresponding to internal sequence amino acids 260-270 of Human PTCHD1 (NP_775766.2).

  • 阳性对照
    • Human Brain (Cerebellum) lysate.

性能

应用

Our Abpromise guarantee covers the use of ab109407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).

靶标

图片

  • Anti-PTCHD1 antibody (ab109407) at 0.5 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.

实验方案

文献

ab109407 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (cerebellum, kidney)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
70 µg
Specification
cerebellum, kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mike Liu

Verified customer

提交于 Apr 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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