Anti-PTBP1抗体[SH54] (ab30317)

概述

  • 产品名称Anti-PTBP1抗体[SH54]
    参阅全部 PTBP1 一抗
  • 描述
    小鼠单克隆抗体[SH54] to PTBP1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    HeLa nuclear extract (Human)

  • 阳性对照
    • IHC-P: human breast carcinoma WB: Jurkat cell lysate, HeLa nuclear lysate ICC: Jurkat, HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab30317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • 序列相似性Contains 4 RRM (RNA recognition motif) domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 57 kDa RNA binding protein PPTB 1 antibody
    • 57 kDa RNA-binding protein PPTB-1 antibody
    • Heterogeneous nuclear ribonucleoprotein I antibody
    • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
    • hnRNP I antibody
    • HNRNP-I antibody
    • HNRNPI antibody
    • HNRPI antibody
    • MGC10830 antibody
    • MGC8461 antibody
    • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
    • Polypyrimidine Tract Binding Protein 1 antibody
    • Polypyrimidine tract binding protein antibody
    • Polypyrimidine tract-binding protein 1 antibody
    • pPTB antibody
    • PTB 1 antibody
    • PTB 2 antibody
    • PTB 3 antibody
    • PTB 4 antibody
    • PTB antibody
    • PTB T antibody
    • PTB1 antibody
    • PTB2 antibody
    • PTB3 antibody
    • PTB4 antibody
    • PTBP 1 antibody
    • PTBP1 antibody
    • PTBP1_HUMAN antibody
    • PTBT antibody
    • RNA Binding Protein antibody
    see all

Anti-PTBP1 antibody [SH54] 图像

  • Overlay histogram showing HeLa cells stained with ab30317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30317, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-PTBP1 antibody [SH54] (ab30317)参考文献

This product has been referenced in:
  • Cosme RS  et al. Roles of polypyrimidine tract binding proteins in major immediate-early gene expression and viral replication of human cytomegalovirus. J Virol 83:2839-50 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19144709) »
  • Huang S  et al. The perinucleolar compartment and transcription. J Cell Biol 143:35-47 (1998). Read more (PubMed: 9763419) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. The antibody was raised in mice immunized with HeLa nuclear protein extract as described and shown in this publication: http://www.jcb.org/cgi/content/full/137/5/965 It's a protein A purified mouse monoclonal IgG1....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Lymphoblast cell line GM10725)
Specification Human Lymphoblast cell line GM10725
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Zeeshan Ali

Verified customer

提交于 Dec 06 2007

I'm sorry to hear you are experiencing problems with ab30317. I have been in contact with the laboratory that tested the antibody and received information which I hope will be useful: PTB expression can be high and at some point during growth and...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"