概述

  • 产品名称Anti-PTBP1抗体
    参阅全部 PTBP1 一抗
  • 描述
    兔多克隆抗体to PTBP1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 508 - 557 (KGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKST I) of Human PTBP1 (NP_002810)

  • 阳性对照
    • Jurkat cell lysate. IF/ICC: HepG2 cell line.

性能

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应用

Our Abpromise guarantee covers the use of ab83897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • 序列相似性Contains 4 RRM (RNA recognition motif) domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 57 kDa RNA binding protein PPTB 1 antibody
    • 57 kDa RNA-binding protein PPTB-1 antibody
    • Heterogeneous nuclear ribonucleoprotein I antibody
    • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
    • hnRNP I antibody
    • HNRNP-I antibody
    • HNRNPI antibody
    • HNRPI antibody
    • MGC10830 antibody
    • MGC8461 antibody
    • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
    • Polypyrimidine Tract Binding Protein 1 antibody
    • Polypyrimidine tract binding protein antibody
    • Polypyrimidine tract-binding protein 1 antibody
    • pPTB antibody
    • PTB 1 antibody
    • PTB 2 antibody
    • PTB 3 antibody
    • PTB 4 antibody
    • PTB antibody
    • PTB T antibody
    • PTB1 antibody
    • PTB2 antibody
    • PTB3 antibody
    • PTB4 antibody
    • PTBP 1 antibody
    • PTBP1 antibody
    • PTBP1_HUMAN antibody
    • PTBT antibody
    • RNA Binding Protein antibody
    see all

Anti-PTBP1 antibody 图像

  • ICC/IF image of ab83897 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83897, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PTBP1 antibody (ab83897) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 43 kDa,45 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab83897 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83897, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PTBP1 antibody (ab83897)参考文献

This product has been referenced in:
  • Clower CV  et al. The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci U S A 107:1894-9 (2010). WB ; Human . Read more (PubMed: 20133837) »

See 1 Publication for this product

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