The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
ab118818, at 5 µg/ml, staining PTBP1 in formalin-fixed, paraffin-embedded Human Small Intestine tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab118818, at 5 µg/ml, staining PTBP1 in formalin-fixed, paraffin-embedded Human Skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-PTBP1 antibody (ab118818)
Anti-PTBP1 antibody (ab118818) at 1 µg/ml + Jurkat lysate (in RIPA buffer) at 30 µg Developed using the ECL technique
Predicted band size : 57 kDa
Anti-PTBP1 antibody (ab118818)参考文献
has not yet been referenced specifically in any publications.