概述

  • 产品名称Anti-PSTPIP1抗体
    参阅全部 PSTPIP1 一抗
  • 描述
    小鼠单克隆抗体to PSTPIP1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length Human protein (AAH08602)

  • 阳性对照
    • Raw 264.7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab88474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Involved in regulation of the actin cytoskeleton. May regulate the WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to the ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allows PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2.
  • 组织特异性Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta.
  • 疾病相关Defects in PSTPIP1 are the cause of PAPA syndrome (PAPAS) [MIM:604416]; also known as pyogenic sterile arthritis, pyoderma gangrenosum and acne or familial recurrent arthritis (FRA). PAPAS is characterized by autosomal dominant inheritance of early onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction.
  • 序列相似性Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • 结构域The coiled domain mediates interaction with PTPN18, PTPN12 and CD2AP. The SH3 domain mediates interaction with WAS and ABL1 (By similarity). The SH3 and coiled-coil domains are necessary for the interaction with MEFV.
  • 翻译后修饰Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cytoplasm > perinuclear region. Cleavage furrow. Colocalized with the cortical actin cytoskeleton during interphase, lamellipodia and actin-rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD2 antigen binding protein 1 antibody
    • CD2 binding protein 1 antibody
    • CD2 cytoplasmic tail binding protein antibody
    • CD2-binding protein 1 antibody
    • CD2BP1 antibody
    • CD2BP1L antibody
    • CD2BP1S antibody
    • H PIP antibody
    • H-PIP antibody
    • HPIP antibody
    • PAPAS antibody
    • PEST phosphatase interacting protein 1 antibody
    • PEST phosphatase-interacting protein 1 antibody
    • PPIP1_HUMAN antibody
    • Proline serine threonine phosphatase interacting protein 1 antibody
    • Proline-serine-threonine phosphatase-interacting protein 1 antibody
    • PSTPIP antibody
    • Pstpip1 antibody
    see all

Anti-PSTPIP1 antibody 图像

  • Anti-PSTPIP1 antibody (ab88474) at 1 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 48 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • Anti-PSTPIP1 antibody (ab88474) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 48 kDa

Anti-PSTPIP1 antibody (ab88474)参考文献

ab88474 has not yet been referenced specifically in any publications.

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