Anti-PSTAIR抗体[PSTAIR] (ab10345)

概述

  • 产品名称Anti-PSTAIR抗体[PSTAIR]
  • 描述
    小鼠单克隆抗体[PSTAIR] to PSTAIR
  • 经测试应用适用于: ELISA, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Monkey, Plants, Quail
    预测可用于: Fish, a wide range of other species
  • 免疫原

    Synthetic peptide:

    EGVPSTAIREISLLKE

    conjugated to BSA, corresponding to amino acids 42-57 of Human PSTAIR.

  • 表位Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).

性能

应用

Our Abpromise guarantee covers the use of ab10345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IP
WB
ICC/IF
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (PMID 19187565).
    IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.
    WB: 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.

    Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The cell cycle is controlled in part by cyclin dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
    • 别名
      • EGVPSTAIREISLLKE antibody
      • PSTAIR motif antibody
      • PSTAIR sequence antibody
      • PSTAIRE antibody

    Anti-PSTAIR antibody [PSTAIR] 图像

    • All lanes : Anti-PSTAIR antibody [PSTAIR] (ab10345) at 1/6000 dilution

      Lane 1 : Whole cell lysate from wild-type S. cerevisiae yeast cells
      Lane 2 : Supernatant after incubation with Protein A-Sepharose beads
      Lane 3 : Eluate from Protein A-Sepharose beads after treatment with TEV protease
      Lane 4 : Product of boiling Protein A-Sepharose after TEV treatment

      Secondary
      Goat anti-mouse HRP conjugate
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 31 kDa
      Observed band size : 32,34 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      See Abreview

    • ab10345 staining PSTAIR in Porcine Ooocytes by Immunocytochemistry/ Immunofluorescence. Oocytes were partially denuded by gentle pipettting in PEM-PVPc (80 mM PIPES, 5mM EGTA. 2 mM MgCl2, pH 6.8, supplemented with 0.3% (w/v) PVP) supplemented with either 0.01% (w/v) pronase (for < 22 h cultured oocytes), or 0.1% (w/v) hyaluronidase (for ≥ 22 h cultured oocytes). After washing in PEM-PVP at 37°C, oocytes were fixed in freshly prepared PEM-PVP containing 4% (v/v) paraformaldehyde at room temperature (RT) for 1 h. An Alexa Fluor® 488 conjugated Goat anti mouse IgG was used as secondary. ab10345 show green PSTAIR staining and DNA was stained blue. Arrow indicate the area that is shown enlarged in the inset. Left image show stainming with ab10345 and right one show staining with mouse IgG as negative control.

    Anti-PSTAIR antibody [PSTAIR] (ab10345)参考文献

    This product has been referenced in:
    • Anderson MZ  et al. Silencing is noisy: population and cell level noise in telomere-adjacent genes is dependent on telomere position and sir2. PLoS Genet 10:e1004436 (2014). WB . Read more (PubMed: 25057900) »
    • Wahdan-Alaswad RS  et al. Metformin-Induced Killing of Triple-Negative Breast Cancer Cells Is Mediated by Reduction in Fatty Acid Synthase via miRNA-193b. Horm Cancer 5:374-89 (2014). Read more (PubMed: 25213330) »

    See all 9 Publications for this product

    Product Wall

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    1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

    or ab96879; http:...

    Read More

    Thank you very much for contacting us.

    We recommend making small aliquots e.g. 10ul or 5 ul each when storing the antibody. For extra stability glycerol at 1:1 ration can be added.

    I hope this information will be helpful. Should you h...

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    Thank you for your enquiry. I am sorry but we do not have a western image of ab20994. However, I have received an image of ab24702 applied using wild type yeast strains and a temperature sensitive strain. I have uploaded it to the antibody datashee...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5%
    Username

    Mr. Mark Chee

    Verified customer

    提交于 Jan 27 2006

    Thank you for your enquiry. This antibody cross-reacts with Human, Mouse, Rat, Xenopus laevis, Cow, Fish, Monkey, Plants, Quail and a wide range of other species. It thus should detect PSTAIR in Arabidopsis. However, I do not know if PSTAIR is ubiquito...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"