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Our Abpromise guarantee covers the use of ab10345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab10345 staining PSTAIR in Porcine Ooocytes by Immunocytochemistry/ Immunofluorescence. Oocytes were partially denuded by gentle pipettting in PEM-PVPc (80 mM PIPES, 5mM EGTA. 2 mM MgCl2, pH 6.8, supplemented with 0.3% (w/v) PVP) supplemented with either 0.01% (w/v) pronase (for < 22 h cultured oocytes), or 0.1% (w/v) hyaluronidase (for ≥ 22 h cultured oocytes). After washing in PEM-PVP at 37°C, oocytes were fixed in freshly prepared PEM-PVP containing 4% (v/v) paraformaldehyde at room temperature (RT) for 1 h. An Alexa Fluor® 488 conjugated Goat anti mouse IgG was used as secondary. ab10345 show green PSTAIR staining and DNA was stained blue. Arrow indicate the area that is shown enlarged in the inset. Left image show stainming with ab10345 and right one show staining with mouse IgG as negative control.