Anti-PSPC1抗体(ab115184)
Key features and details
- Rabbit polyclonal to PSPC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PSPC1抗体
参阅全部 PSPC1 一抗 -
描述
兔多克隆抗体to PSPC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Chinese hamster, Bat, Elephant -
免疫原
Synthetic peptide, corresponding to a region within amino acids 245-275 of Human PSPC1 (NP_001035879.1).
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阳性对照
- HeLa, 293T or Jurkat whole cell lysate (ab7899).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 59 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. -
组织特异性
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. -
序列相似性
Belongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps. - Information by UniProt
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数据库链接
- Entrez Gene: 55269 Human
- Entrez Gene: 66645 Mouse
- Entrez Gene: 305910 Rat
- Omim: 612408 Human
- SwissProt: Q8WXF1 Human
- SwissProt: Q8R326 Mouse
- SwissProt: Q4KLH4 Rat
- Unigene: 213198 Human
see all -
别名
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
see all
图片
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All lanes : Anti-PSPC1 antibody (ab115184) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 3 minutes -
Samples: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
Antibodies: ab115184 used for WB at 0.4 µg/ml and used for IP at 6 µg/mg lysate.
Detection: chemiluminescence with exposure time of 30 seconds (B).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab115184 被引用在 1 文献中.
- Wang J et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). PubMed: 28192372