概述

  • 产品名称Anti-PSMD11抗体
    参阅全部 PSMD11 一抗
  • 描述
    兔多克隆抗体to PSMD11
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 372-422 of Human PSMD11 ((NP_002806.2).

  • 阳性对照
    • Human HeLa and 293T cell lines. Mouse NIH3T3 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • 组织特异性Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
  • 序列相似性Belongs to the proteasome subunit S9 family.
    Contains 1 PCI domain.
  • 翻译后修饰Phosphorylated by AMPK.
  • 细胞定位Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S proteasome non-ATPase regulatory subunit 11 antibody
    • 26S proteasome regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p44.5 antibody
    • 26S proteasome regulatory subunit RPN6 antibody
    • 26S proteasome regulatory subunit S9 antibody
    • MGC3844 antibody
    • p44.5 antibody
    • protease 26S, subunit, 9 antibody
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 antibody
    • proteasome 26S subunit, non-ATPase, 11 antibody
    • PSD11_HUMAN antibody
    • PSMD 11 antibody
    • PSMD11 antibody
    • Rpn6 antibody
    • S9 antibody
    see all

Anti-PSMD11 antibody 图像

  • All lanes : Anti-PSMD11 antibody (ab99414) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99414 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: PSMD11 antibody (ab99414) at 0.4 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

    Predicted band size : 47 kDa

Anti-PSMD11 antibody (ab99414)参考文献

ab99414 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99414.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"