概述

  • 产品名称
  • 描述
    兔多克隆抗体to PSMD11
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 372-422 of Human PSMD11 ((NP_002806.2).

  • 阳性对照
    • Human HeLa and 293T cell lines. Mouse NIH3T3 cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • 组织特异性
    Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
  • 序列相似性
    Belongs to the proteasome subunit S9 family.
    Contains 1 PCI domain.
  • 翻译后修饰
    Phosphorylated by AMPK.
  • 细胞定位
    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S proteasome non-ATPase regulatory subunit 11 antibody
    • 26S proteasome regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p44.5 antibody
    • 26S proteasome regulatory subunit RPN6 antibody
    • 26S proteasome regulatory subunit S9 antibody
    • MGC3844 antibody
    • p44.5 antibody
    • protease 26S, subunit, 9 antibody
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 antibody
    • proteasome 26S subunit, non-ATPase, 11 antibody
    • PSD11_HUMAN antibody
    • PSMD 11 antibody
    • PSMD11 antibody
    • Rpn6 antibody
    • S9 antibody
    see all

图片

  • All lanes : Anti-PSMD11 antibody (ab99414) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99414 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: PSMD11 antibody (ab99414) at 0.4 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

    Predicted band size : 47 kDa

文献

ab99414 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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