概述

  • 产品名称Anti-PSMD11抗体
    参阅全部 PSMD11 一抗
  • 描述
    兔多克隆抗体to PSMD11
  • 经测试应用适用于: IHC-Fr, IP, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Zebrafish
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Saccharomyces cerevisiae
  • 免疫原

    A region within synthetic peptide: CVAQASKNRS LADFEKALTD YRAELRDDPI ISTHLAKLYD NLLEQNLIRV, corresponding to C terminal amino acids 289-338 of Human PSMD11

  • 阳性对照
    • Fetal brain lysate. Human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab66346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • 组织特异性Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
  • 序列相似性Belongs to the proteasome subunit S9 family.
    Contains 1 PCI domain.
  • 翻译后修饰Phosphorylated by AMPK.
  • 细胞定位Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S proteasome non-ATPase regulatory subunit 11 antibody
    • 26S proteasome regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p44.5 antibody
    • 26S proteasome regulatory subunit RPN6 antibody
    • 26S proteasome regulatory subunit S9 antibody
    • MGC3844 antibody
    • p44.5 antibody
    • protease 26S, subunit, 9 antibody
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 antibody
    • proteasome 26S subunit, non-ATPase, 11 antibody
    • PSD11_HUMAN antibody
    • PSMD 11 antibody
    • PSMD11 antibody
    • Rpn6 antibody
    • S9 antibody
    see all

Anti-PSMD11 antibody 图像

  • Anti-PSMD11 antibody (ab66346) at 5 µg/ml + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-PSMD11 antibody (ab66346)

  • ab66346 staining PSMD11 in Human colorectal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes at 16°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/25 in 1.5% Normal Horse serum) for 2 hours at 16°C. An undiluted Biotin-conjugated Human pan-specific polyclonal was used as the secondary antibody.

    See Abreview

Anti-PSMD11 antibody (ab66346)参考文献

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - tritonx-100
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Jun 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (brain)
Total protein in input 40 µg
Specification brain
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal Cancer)
Specification Colorectal Cancer
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 16°C
Username

Dr. LUCY SCAIFE

Verified customer

提交于 Mar 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Oral Cancer)
Loading amount 20 µg
Specification Oral Cancer
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"