概述

  • 产品名称Anti-PSMD1抗体
    参阅全部 PSMD1 一抗
  • 描述
    兔多克隆抗体to PSMD1
  • 特异性Detects proteasome Rpn2 from human cells.
  • 经测试应用适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Cow, Human, Non Human Primates
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide corresponding to Human PSMD1 aa 929-953.
    Sequence:

    AAHGPKIEEEEQEPEPPEPFEYIDD

  • 阳性对照
    • HeLa cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab2941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.

IHC-Fr Use a concentration of 15 µg/ml.
IHC-P Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-PSMD1 antibody 图像



  • Predicted band size : 105 kDa

    Western blot of proteasome Rpn2 from HeLa cell extract using ab2941.

  • ab2941 (4µg/ml) staining Rpn2 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei and cytoplasm within the sebaceous gland.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab2941 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PSMD1 antibody (ab2941)参考文献

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Thank you for your phone call. The concentration is 1 mg/ml. If you have any additional questions, please let us know. Have a great weekend!

Thanks for your enquiry. To our knowledge, this antibody has yet to be tested in IP. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in retu...

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All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"