概述

  • 产品名称Anti-PSMD1抗体
    参阅全部 PSMD1 一抗
  • 描述
    小鼠多克隆抗体to PSMD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    IKETKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRK FKGNNGVVVLRDREPKEPVALIETVRQMKDVNAPLPTPFKVDDNVDFPSA

    , corresponding to amino acids 846/945 of Saccharomyces cerevisiae Rpn2

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 104 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • 序列相似性Belongs to the proteasome subunit S1 family.
    Contains 10 PC repeats.
  • Information by UniProt
  • 别名
    • MGC133041 antibody
    • 26S proteasome non ATPase regulatory subunit 1 antibody
    • 26S proteasome non ATPase subunit 1 antibody
    • 26S proteasome non-ATPase regulatory subunit 1 antibody
    • 26S proteasome regulatory subunit rpn2 antibody
    • 26S proteasome regulatory subunit S1 antibody
    • 26S proteasome subunit p112 antibody
    • MGC133040 antibody
    • P112 antibody
    • Proteasome (prosome macropain) 26S subunit, non ATPase 1 antibody
    • Psmd1 antibody
    • PSMD1_HUMAN antibody
    • RPN2 antibody
    • S1 antibody
    see all

Anti-PSMD1 antibody 图像

  • All lanes : Anti-PSMD1 antibody (ab21638) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 104 kDa

Anti-PSMD1 antibody (ab21638)参考文献

This product has been referenced in:
  • Kuzina E  et al. Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome. Biomed Res Int 2014:926394 (2014). WB . Read more (PubMed: 25276831) »

See 1 Publication for this product

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