概述

  • 产品名称Anti-PSMC5抗体
    参阅全部 PSMC5 一抗
  • 描述
    兔多克隆抗体to PSMC5
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human PSMC5 (NP_002796.4)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

靶标

  • 功能The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
  • 序列相似性Belongs to the AAA ATPase family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S protease regulatory subunit 8 antibody
    • 26S proteasome AAA-ATPase subunit RPT6 antibody
    • Cim3 antibody
    • MSUG1 protein antibody
    • p45 antibody
    • p45/SUG antibody
    • Proteasome 26S ATPase subunit 5 antibody
    • Proteasome 26S subunit ATPase 5 antibody
    • Proteasome prosome macropain 26S subunit ATPase 5 antibody
    • Proteasome subunit p45 antibody
    • PRS8_HUMAN antibody
    • PSMC5 antibody
    • Rpt6 antibody
    • S8 antibody
    • SUG1 antibody
    • Tat binding protein homolog 10 antibody
    • TBP10 antibody
    • Thyroid hormone receptor interacting protein 1 antibody
    • Thyroid hormone receptor-interacting protein 1 antibody
    • Thyroid receptor interactor 1 antibody
    • TRIP1 antibody
    • TRIP1(SUG1) antibody
    see all

Anti-PSMC5 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling PSMC5 with ab70832 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PSMC5 antibody (ab70832) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 30 seconds
  • Detection of Human PSMC5 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells, using ab70832 at 3 µg/mg lysate (Lane 1). Lane 2 represents control IgG IP. Subsequent WB detection was performed using 1 µg/ml ab70832 and chemiluminescence with an exposure time of 30 seconds.

Anti-PSMC5 antibody (ab70832)参考文献

ab70832 has not yet been referenced specifically in any publications.

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