Mouse, Rat, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 51-331 of Human PSMC3 (NP_002795).
293T, A431, H1299, HeLa, HepG2, Raji cell lysates; Molt-4 whole cell lysate (WB); A549 cells (IF); Cal27 xenograft (IHC)
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
1/100 - 1/500.
1/100 - 1/200.
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Belongs to the AAA ATPase family.
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
Information by UniProt
26S protease regulatory subunit 6A antibody
26S proteasome AAA-ATPase subunit RPT5 antibody
Human immunodeficiency virus tat transactivator binding protein 1 antibody
Western blot - PSMC3 antibody (ab96558)
Anti-PSMC3 antibody (ab96558) at 1/5000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size : 49 kDa
Immunocytochemistry/ Immunofluorescence - PSMC3 antibody (ab96558)
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab96558 at 1/200 dilution. Lower image merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMC3 antibody (ab96558)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96558 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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