概述

  • 产品名称Anti-PSMB5抗体
    参阅全部 PSMB5 一抗
  • 描述
    兔多克隆抗体to PSMB5
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human PSMB5 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P28074
    (Peptide available as ab101292, ab101292)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Liver Tissue; Mouse Liver Tissue; Mouse Brain Tissue; HepG2 Whole Cell Lysate; HeLa Whole Cell Lysate; Jurkat Whole Cell Lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 29 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway.
  • 序列相似性Belongs to the peptidase T1B family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp459C139 antibody
    • EC 3.4.25.1 antibody
    • LMPX antibody
    • Macropain epsilon chain antibody
    • MB1 antibody
    • MGC104214 antibody
    • MGC118075 antibody
    • MGC134464 antibody
    • Multicatalytic endopeptidase complex epsilon chain antibody
    • Proteasome (prosome, macropain) subunit, beta type, 5 antibody
    • Proteasome beta 5 subunit antibody
    • Proteasome catalytic subunit 3 antibody
    • Proteasome chain 6 antibody
    • Proteasome epsilon chain antibody
    • Proteasome subunit beta type-5 antibody
    • Proteasome subunit MB1 antibody
    • Proteasome subunit X antibody
    • Proteasome subunit, beta type, 5 antibody
    • Proteasome subunit, beta-5 antibody
    • PSB5_HUMAN antibody
    • PSMB5 antibody
    • PSX large multifunctional protease X antibody
    • X antibody
    see all

Anti-PSMB5 antibody 图像

  • All lanes : Anti-PSMB5 antibody (ab90867) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab90867 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90867, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PSMB5 antibody (ab90867)参考文献

This product has been referenced in:
  • Chan NC  et al. Broad activation of the ubiquitin-proteasome system by Parkin is critical for mitophagy. Hum Mol Genet 20:1726-37 (2011). ICC/IF ; Human . Read more (PubMed: 21296869) »

See 1 Publication for this product

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