RabMAb

Anti-PSMA抗体[EP3254] (ab79542)

概述

  • 产品名称Anti-PSMA抗体[EP3254]
    参阅全部 PSMA 一抗
  • 描述
    兔单克隆抗体[EP3254] to PSMA
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PSMA aa 350-450 (C terminal).

  • 阳性对照
    • LnCap lysate. LnCap cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab79542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 100-120 kDa (predicted molecular weight: 84 kDa).

For unpurified, use 1/1000.

  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
    • 组织特异性Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
    • 序列相似性Belongs to the peptidase M28 family. M28B subfamily.
    • 结构域The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
    • 翻译后修饰The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.
    • 细胞定位Cytoplasm and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell growth inhibiting protein 27 antibody
      • Cell growth-inhibiting gene 27 protein antibody
      • FGCP antibody
      • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
      • Folate hydrolase 1 antibody
      • Folate hydrolase antibody
      • Folate hydrolase prostate specific membrane antigen 1 antibody
      • FOLH 1 antibody
      • FOLH antibody
      • Folh1 antibody
      • FOLH1_HUMAN antibody
      • Folylpoly gamma glutamate carboxypeptidase antibody
      • Folylpoly-gamma-glutamate carboxypeptidase antibody
      • GCP 2 antibody
      • GCP II antibody
      • GCP2 antibody
      • GCPII antibody
      • GIG27 antibody
      • Glutamate carboxylase II antibody
      • Glutamate carboxypeptidase 2 antibody
      • Glutamate carboxypeptidase II antibody
      • Membrane glutamate carboxypeptidase antibody
      • mGCP antibody
      • N acetylated alpha linked acidic dipeptidase 1 antibody
      • N-acetylated-alpha-linked acidic dipeptidase I antibody
      • NAALAD 1 antibody
      • NAALAD1 antibody
      • NAALAdase antibody
      • NAALADase I antibody
      • Prostate specific membrane antigen antibody
      • Prostate specific membrane antigen variant F antibody
      • Prostate-specific membrane antigen antibody
      • PSM antibody
      • PSMA antibody
      • Pteroylpoly gamma glutamate carboxypeptidase antibody
      • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
      see all

    Anti-PSMA antibody [EP3254] 图像

    • Anti-PSMA antibody [EP3254] (ab79542) at 1/3000 dilution (purified) + LnCaP cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 84 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-PSMA antibody [EP3254] (ab79542) at 1/5000 dilution (unpurified) + LnCap lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 84 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Anti-PSMA antibody [EP3254] (ab79542)参考文献

    ab79542 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for sending in the sequence. I have blasted the sequence of the dog protein to human and it shows 98% homology.

    Yes, the PSMA antibody [EP3254] ab79542 detects the extracellular region of the Human PMSA.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"