• 产品名称
  • 描述
    兔多克隆抗体to PSIP1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: TEQQNKDEGK KPEVKKVEKK RETSMDSRLQ RIHAEIKNSL KIDNLDVNRC, corresponding to internal sequence amino acids 324-373 of Human PSIP1

  • 阳性对照
    • Jurkat cell lysate



Our Abpromise guarantee covers the use of ab49281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.


  • 功能
    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • 组织特异性
    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • 疾病相关
    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • 序列相似性
    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • 结构域
    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLL associated antigen KW 7 antibody
    • CLL-associated antigen KW-7 antibody
    • Dense fine speckles 70 kDa protein antibody
    • DFS 70 antibody
    • DFS70 antibody
    • LEDGF antibody
    • Lens epithelium derived growth factor antibody
    • Lens epithelium-derived growth factor antibody
    • MGC74712 antibody
    • p52 antibody
    • p75 antibody
    • PAIP antibody
    • PC4 and SFRS1 interacting protein 1 antibody
    • PC4 and SFRS1 interacting protein 2 antibody
    • PC4 and SFRS1 interacting protein antibody
    • PC4 and SFRS1-interacting protein antibody
    • PSIP 1 antibody
    • PSIP 2 antibody
    • Psip1 antibody
    • PSIP1_HUMAN antibody
    • PSIP2 antibody
    • Transcriptional coactivator p75/p52 antibody
    see all

Anti-PSIP1 antibody 图像

  • Predicted band size : 60 kDa
    Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.

  • Predicted band size : 60 kDa
    Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.
  • Anti-PSIP1 antibody (ab49281) at 5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%


Anti-PSIP1 antibody (ab49281)参考文献

ab49281 has not yet been referenced specifically in any publications.

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Thank you very much for your interest in ab49281. To our knowledge, ab49281 has not been tested using a Biacore. It is not possible to submit Abreviews for this application but we would be interested in your experience with IP. Therefore, I can...

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