概述

  • 产品名称Anti-PSG9抗体
    参阅全部 PSG9 一抗
  • 描述
    兔多克隆抗体to PSG9
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: VYSNASLLIQ NVTRKDAGTY TLHIIKRGDE TREEIRHFTF TLYLETPKPY , corresponding to amino acids 101-150 of Human PSG9

  • 阳性对照
    • HepG2 cell lysate,

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 序列相似性Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 发展阶段PSBG are produced in high quantity during pregnancy.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • pregnancy specific beta 1 glycoprotein (PSG) antibody
    • pregnancy specific beta 1 glycoprotein 11 antibody
    • pregnancy specific beta 1 glycoprotein 9 antibody
    • pregnancy specific beta 1 glycoprotein B antibody
    • Pregnancy-specific beta-1 glycoprotein B antibody
    • Pregnancy-specific beta-1-glycoprotein 11 antibody
    • Pregnancy-specific beta-1-glycoprotein 9 antibody
    • Pregnancy-specific glycoprotein 11 antibody
    • Pregnancy-specific glycoprotein 7 antibody
    • Pregnancy-specific glycoprotein 9 antibody
    • PS beta B antibody
    • PS-beta-B antibody
    • PS-beta-G-11 antibody
    • PS-beta-G-9 antibody
    • PS34 antibody
    • PSBG 9 antibody
    • PSBG-11 antibody
    • PSBG-9 antibody
    • PSG 9 antibody
    • PSG11 antibody
    • PSG7 antibody
    • PSG9 antibody
    • PSG9_HUMAN antibody
    • PSGII antibody
    see all

Anti-PSG9 antibody 图像

  • Anti-PSG9 antibody (ab64425) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab64425 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64425, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PSG9 antibody (ab64425)参考文献

ab64425 has not yet been referenced specifically in any publications.

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