Anti-PSD95 (phospho S295)抗体(ab16495)

概述

  • 产品名称Anti-PSD95 (phospho S295)抗体
    参阅全部 PSD95 一抗
  • 描述
    兔多克隆抗体to PSD95 (phospho S295)
  • 特异性PSD95 has a predicted band size of 95kDa, however in western blot gels it tends to run at a larger band size (~105kDa), this is thought to be due to post-translational modification (possibly palmitoylation) of PSD95 proteins.
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse PSD95, phosphorylated at S295.

    (Peptide available as ab17222.)

  • 阳性对照
    • mouse hippocampus

性能

应用

Our Abpromise guarantee covers the use of ab16495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/300.
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 95 kDa).

靶标

  • 功能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 组织特异性Brain.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻译后修饰Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 细胞定位Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 (phospho S295) antibody 图像

  • Lane 1 - Input lane, 25-50µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)

    Lane 2 - IP lane, ~500µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)

    The IP lane (2) shows an enrichment of PSD95 (phospho S295) at the expected band size (~105kDa).

  • Immunohistochemistical detection of PSD95 (phospho S295) using antibody ab16495 on PFA perfusion-fixed mouse brain sections (30 microns). Primary antibody ab16495 was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000). The image show immunostaining obtained in the mouse cerebellum using this antibody using direct fluorescence. The antibody stained granular cerebellar cells. Image [A] shows the staining obtained using the antibody, whereas image [B] is the staining captured with the same setup of acquisition for the negative control (lack of primary antibody). The arrows show the location of purkinje cells.

    See Abreview

Anti-PSD95 (phospho S295) antibody (ab16495)参考文献

ab16495 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (hippocampus)
Loading amount 5 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (4–12% Bis–Tris NuPAGE gradient gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Spinal cord lysate)
Loading amount 30 µg
Specification Spinal cord lysate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

提交于 Aug 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"