Anti-PSD95抗体[S28-43] (ab99009)

概述

  • 产品名称Anti-PSD95抗体[S28-43]
    参阅全部 PSD95 一抗
  • 描述
    小鼠单克隆抗体[S28-43] to PSD95
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Fr, Electron Microscopymore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 77-200 of Human PSD95

  • 阳性对照
    • Rat brain tissue extract

性能

应用

Our Abpromise guarantee covers the use of ab99009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration. (Immuno-gold). Sufficient for detection of PSD95 in 20 µg of rat brain tissue extract using Goat anti-mouse IgG:HRP as the secondary antibody.

靶标

  • 功能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 组织特异性Brain.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻译后修饰Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 细胞定位Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 antibody [S28-43] 图像

  • Immunofluorescent detection of PSD95 using ab99009 in cultures of dissociated hippocampal neurons.
  • Immunolabelling of PSD95 using a dilution of ab99009 in Mouse cerebellum sections. Specific labelling of the puncta below the Purkinje cells of the cerebellum is seen in this image.

Anti-PSD95 antibody [S28-43] (ab99009)参考文献

This product has been referenced in:
  • Ji B  et al. Inhibition of protein translation by the DISC1-Boymaw fusion gene from a Scottish family with major psychiatric disorders. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24908665) »

See 1 Publication for this product

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Thank you very much for contacting us with your question.

This code did expire on March 23, 2012.However, I've justextended the expiration date for this code, and it needs to be used before July 12, 2012 (one week from today) so please have t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (Brain)
Loading amount 23 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10% resolving SDS-PAGE gel)
Blocking step Milk as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Mrs. Fusun Doldur Balli

Verified customer

提交于 Apr 02 2012

Thank you for contacting me and letting me know about this situation.

I have entered your Abreview codes into the corresponding reviews, so these are now active and can be redeemed for free primary antibodies.

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Thank you for contacting us and for your patience while I have reviewed the situation. I am sorry to see that the resultshave not been optimal with these antibodies, but I am hopeful that we can improve the blots.

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