概述

  • 产品名称Anti-PSD95抗体
    参阅全部 PSD95 一抗
  • 描述
    兔多克隆抗体to PSD95
  • 经测试应用适用于: IHC-FoFr, ICC/IF, WB, IHC-P, ICC, IHC-Fr, Sandwich ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish, Cynomolgus Monkey, Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse PSD95.

    (Peptide available as ab18661.)

  • 阳性对照
    • ab18258 gave a positvie result in the following Tissue Lysates Mouse Brain Rat Brain

性能

应用

Our Abpromise guarantee covers the use of ab18258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20874778
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

Abcam recommends using 3% milk as the blocking agent.

IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr 1/1000. PubMed: 17959791
Sandwich ELISA Use at an assay dependent concentration. PubMed: 20169337Used as detection antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 组织特异性Brain.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻译后修饰Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 细胞定位Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 antibody 图像

  • ab18258 staining PSD95 in Zebrafish retina (inner plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab18258 (1/1000) staining PSD9 in paraffin-embedded rat cerebellum. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 mins/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin. For further experimental details, please refer to abreview.

    See Abreview

  • IHC image of PSD95 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18258, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-PSD95 antibody (ab18258) at 1/350 dilution

    Lane 1 : Marmoset (common) brain whole tissue lysate
    Lane 2 : Marmoset (common) brain whole tissue lysate
    Lane 3 : Marmoset (common) brain whole tissue lysate
    Lane 4 : Marmoset (common) brain whole tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/12000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 80 kDa


    Exposure time : 16 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-PSD95 antibody (ab18258) at 1 µg/ml

    Lane 1 : wildtype mouse brain tissue lysate
    Lane 2 : PSD95 knockout mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : ~80 kDa (why is the actual band size different from the predicted?)
  • Lane 1 - Input lane 500ug mouse brain lysate

    Lane 2 - IP lane 50ug mouse brain lysate

    NB: as expected, an enrichment of PSD95 protein is observed in the IP lane.

  • All lanes : Anti-PSD95 antibody (ab18258) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 2 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab18258 (1/500) staining PSD95 in paraffin-embedded Mouse Cerebellum (Top) and Medulla (Bottom) tissue, showing positive staining to the synaptic regions of the brain. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 mins/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin.  For further experimental details, please refer to abreview.

    See Abreview

  • ab18258 staining PSD95 in murine retinal tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • ab18258 staining PSD95 in rat retinal tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • ab18258 staining PSD95 in human SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, blocked with 10% serum for 20 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    See Abreview

  • ICC/IF image of ab18258 stained human SHSY5Y cells. The cells were 4% PFA fixed (10 min), permeabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab18258, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-PSD95 antibody (ab18258)参考文献

This product has been referenced in:
  • Wang D & Mitchell ES Cognition and Synaptic-Plasticity Related Changes in Aged Rats Supplemented with 8- and 10-Carbon Medium Chain Triglycerides. PLoS One 11:e0160159 (2016). WB ; Rat . Read more (PubMed: 27517611) »
  • Atzpodien EA  et al. Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs. Toxicol Pathol 44:398-413 (2016). Read more (PubMed: 26680760) »

See all 33 Publications for this product

Product Wall

Application Immunohistochemistry free floating
Sample Zebra finch Tissue sections (Brain)
Specification Brain
Username

Abcam user community

Verified customer

提交于 Sep 15 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 09 2016

Application Western blot
Sample Human Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 09 2016

Application Immunocytochemistry
Sample Mouse Cultured Cells (cortical neurons primary culture)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification cortical neurons primary culture
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 01 2016

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Specification Cryopreserved embryonic cortical neurons (QBMcells
Permeabilization No
Fixative paraformaldehyde with picric acid
Username

Ms. Babben Tinner

Verified customer

提交于 Mar 04 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (retina, inner plexiform layer)
Specification retina, inner plexiform layer
Permeabilization Yes - Triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

提交于 Oct 02 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Sample Marmoset (common) Tissue lysate - whole (brain)
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 23 2014


The antibodies mentioned above are suitable for Rat as well as human samples. ab9018 is yet to be tested in IHC-Fr however as it is tested in IHC-FoFr so it is more likely to work.

The suitable secondary's are for ab4648, ab82259 and ab90...

Read More
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Tissue lysate - whole (mouse brain)
Specification mouse brain
Total protein in input 200 µg
Username

Abcam user community

Verified customer

提交于 Jun 04 2013

1-10 of 46 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"