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Our Abpromise guarantee covers the use of ab12093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
PSD95 (ab12093) western blot on mouse brain extract (10
Lane 1 = 0.3
Lane 2 = 1
Lane 3 = 3
Lane 4 = 3
Lane 5 = Ladder
ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spines
This image is courtesy of an Abreview submitted by Dr Martin BroadstockSee abreview for further details