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Our Abpromise guarantee covers the use of ab12093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
Abcam recommends using milk as the blocking agent.
|IHC-P||Use a concentration of 10 µg/ml.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk before being incubated with ab12093 overnight at 4°C. Antibody binding was detected using an anti-goat antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spines
This image is courtesy of an Abreview submitted by Dr Martin BroadstockSee abreview for further details
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
PSD95 (ab12093) western blot on mouse brain extract (10
Lane 1 = 0.3
Lane 2 = 1
Lane 3 = 3
Lane 4 = 3
Lane 5 = Ladder